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Have you ever wanted to grow mould and bacteria? Have you ever wanted to see the interactions between a mould and Bacteria. Well you have come to the right site. Aim: To grow the mould penicillium. To grow different bacteria’s. Then add them together and see what the interactions are. Hypothesis: The mould should finish growing in about two weeks and the bacteria a little longer. The mould and bacteria should grow at a steady rate with the odd growth spurt. The mould and bacteria should be able to live together until food resources run out. Procedure: Materials needed: · Incubator · Dishwashing liquid · Warm tap water · 1 litre of apple cider · Magnifying glass/Microscope · 17x Petri dishes · Agar · A table surface · Tripod · Bunsen Burner (and gas) · Gauze · Stirring rod · Stock cube (beef extract) · Fridge To grow the mould Penicillium 1: Wash the jar with warm, soapy water and rinse with warm water. 2: Fill the beaker with 250ml of apple cider 3: Place the beaker full of apple cider in a warm and dark place but the temperature must be constant (one variable) 4: Use the magnifying glass/microscope to make daily observations of the surface for two weeks. To grow the Bacteria 1: Again clean the petrie dishes with warm water. 2: Fill petri dishes with agar 3: Innoculate by rubbing over a bable top surface 4: Incubate at 32 degrees celcious and it must stay on this temperature (one variable) 5: Use the magnifying glass/microscope to makedaily observations of the surface for two weeks. Combining the samples: 1: Obtain a Petri dish and will it with agar. 2: Then add the both samples in the Petri dish and make daily observations of the occurring events. 3: Do this for each bacteria and sample of mould. 4: After three weeks determine whether the mould or bacteria still remain and through away the remaining specimens. Time plan: The time plan for this experiment is 24 hours for preparation of materials. Around two weeks to grow the mould and bacterial. Plus another three weeks to determine which specimen still remains. So in total around 29 days. (Plus time to present the information). Results: The penicillium grew in only five days, Well short of the expected twelve days. Below is the day by day break down of the growth rate. Day----------- Size (mm) 1------------ 1 2------------ 4 3----------- 9 4----------- 14 5----------- 20 The bacteria grew in 11 days. One day short of the expected growth time. Below is the day by day growth rate. Day----------- Size (mm) 1------------ 1 2------------- 1 3------------- 3 4------------ 4 5------------ 5 6------------ 7 7------------ 7 8------------ 8.5 9 ----------- 9 10 ---------- 10 11---------- 10 When the bacteria and the penicillium grew together the penicillium killed the bacteria of every type, size and shape. The day by day break down of the size changes are shown below; Bacteria ------------------------ Penicillium Day ----------------Size (mm) Day ----------------Size (mm) 1------------------ 20 ---------- 1------------------ 12 2------------------ 20 ---------- 2------------------ 15 3------------------ 17 ---------- 3------------------ 17 4------------------ 15 ---------- 4------------------ 21 5------------------ 10 ---------- 5------------------ 25 6------------------ 5 ---------- 6------------------ 30 7------------------ 3 ---------- 7------------------ 30 8------------------ 0 ---------- 8------------------ 31 Conclusion The penicillium grew at a very rapid rate and had finished growing in only five days. While the Bacteria grew slow and steady finishing its growing after 11 days. The expected growth times for both samples was 2 weeks, so they grew faster than expected. The interactions between the two samples showed the penicillium killing off all of the bacteria samples. I have proved the point of my hypothesis that the samples would grow at a steady rate with the odd growth spurt. But I have proved the point in my hypothesis that the bacteria and mould could live together until food runs out to be wrong. The mould (penicillium) destroyed all samples of bacteria. The possible uses of my results; * Penicillium used to clean bacteria off kitchen tables. *Penicillium used to kill bacteria inside the human body (penicilan) *Pencillium used to sterilize areas in hospital eg Operating room and equipment.. "Bibliography" All the information I used was from; * Myself * Mr Gaggler * Mr Pigot To inhance the use of this site for the rest of the world it has been put in 29 search engines. These inlude: * Yahoo * Lycos * Searchhound.com * JaydeOnlineDirectory * WhatUseek.com The total cost of this assignment was..... $2 (for the bottle of apple cider)